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Anti-CD200R antibody (bs-10521R)

Anti-CD200R antibody (bs-10521R)

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Anti-CD200R antibody

英文名称CD200R
中文名称CD200受体抗体
别    名CD 200R; CD200 cell surface glycoprotein receptor; CD200 receptor 1; Cd200r1; Cell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; Cell surface glycoprotein OX2 receptor; cell surface glycoprotein receptor CD200; CRTR 2; CRTR2; HCRTR2; MO2R1_HUMAN; MOX2 receptor; MOX2R; OX2R.  

DATASHEET

Host:Rabbit

Target Protein:CD200R

IR:Immunogen Range:201-300/325

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:131450

Swiss Prot:Q8TD46

Source:KLH conjugated synthetic peptide derived from human CD200R:201-300/325 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-CD200R (bs-10521R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD

Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-CD200R (bs-10521R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD200R Polyclonal Antibody, Unconjugated(bs-10521R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

 

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