Anti-NUDCD3 antibody (bs-13766R)

Anti-NUDCD3 antibody

DATASHEET

Host:Rabbit

Target Protein:NUDCD3

IR:Immunogen Range:161-260/361

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:23386

Swiss Prot:Q8IVD9

Source:KLH conjugated synthetic peptide derived from human NUDCD3:161-260/361 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Nuclear migration is essential for growth, development and cellular function of multicellular organisms. NUDCD3 (NudC domain containing 3) is a 361 amino acid protein that contains one CS domain and is phosphorylated upon DNA damage by either ATR or ATM. Ubiquitously expressed, NUDCD3 has been observed to colocalize with Golgi marker proteins during interphase of mitosis in HeLa cells, and is found at low levels in brain, ovary and heart. The gene encoding NUDCD3 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hl-60 Cell (Human) Lysate at 30 ug
Primary: Anti- NUDCD3 (bs-13766R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 50 kD

Sample:
U937 Cell (Human) Lysate at 30 ug
Primary: Anti- NUDCD3 (bs-13766R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 50 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUDCD3) Polyclonal Antibody, Unconjugated (bs-13766R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.