Anti-CHSS2 antibody
| 英文名称 | CHSS2 |
| 中文名称 | 硫酸软骨素酶2抗体 |
| 别 名 | Chondroitin glucuronyltransferase II; Chondroitin polymerizing factor; Chondroitin sulfate synthase 2; CHPF; CHSY2; CSS2; CHSS2_HUMAN; Glucuronosyl N acetylgalactosaminyl proteoglycan 4 beta N acetylgalactosaminyltransferase II; N acetylgalactosaminyl proteoglycan 3 beta glucuronosyltransferase II. |
DATASHEET
Host:Rabbit
Target Protein:CHSS2
IR:Immunogen Range:51-150/775
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:79586
Swiss Prot:Q8IZ52
Source:KLH conjugated synthetic peptide derived from human CHSS2:51-150/775
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:CHSS2 belongs to the chondroitin N-acetylgalactosaminyltransferase family. It has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity and transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. CHSS2 is ubiquitous; highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Siha(human) Cell Lysate at 40 ug
Primary: Anti-CHSS2 (bs-13928R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD
Sample: Mcf-7(human) Cell Lysate at 40 ug
Primary: Anti-CHSS2 (bs-13928R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CHSS2 Polyclonal Antibody, Unconjugated(bs-13928R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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