Anti-Creatine Kinase MB antibody (bs-14049R)

Anti-Creatine Kinase MB antibody

DATASHEET

Host:Rabbit

Target Protein:Creatine Kinase MB

IR:Immunogen Range:281-381/381

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1152

Swiss Prot:P06732

Source:KLH conjugated synthetic peptide derived from human Creatine Kinase MB:281-381/381 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Protein: k562(human) lysate;
Primary: rabbit Anti-Creatine Kinase MB (bs-14049R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 42 kD
Observed band size: 42 kD

Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti-Creatine Kinase MB (bs-14049R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Creatine Kinase MB (bs-14049R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD