Anti-DNTTIP1 antibody (bs-14400R)

Anti-DNTTIP1 antibody 

DATASHEET

Host:Rabbit

Target Protein:DNTTIP1

IR:Immunogen Range:21-120/329

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:116092

Swiss Prot:Q9H147

Source:KLH conjugated synthetic peptide derived from human DNTTIP1:21-120/329 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:DNTTIP1 binds DNA and enhances the activity of terminal deoJNCnucleotidyltransferase (TDT, or DNTT; MIM 187410), a DNA polymerase that catalyzes the polymerization of DNA in the absence of a DNA template (Yamashita et al., 2001 [PubMed 11473582]).[supplied by OMIM, Mar 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
Orangutan.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-DNTTIP1 (bs-14400R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 38 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-DNTTIP1 (bs-14400R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 38 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNTTIP1) Polyclonal Antibody, Unconjugated (bs-14400R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.