Anti-RPS6KA1 antibody
| 英文名称 | RPS6KA1 |
| 中文名称 | 丝氨酸/苏氨酸激酶p90RSK蛋白抗体 |
| 别 名 | RSK1 p90; 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein p90RSK; RSK1 p90; S6 kinase, 90kD, polypeptide 1; dJ590P13.1; EC 2.7.11.1; HU 1; HU1; KS6A1_HUMAN; MAP kinase activated protein kinase 1a; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1A; MAPKAPK-1a; MAPKAPK1A; MGC79981; Mitogen-activated protein kinase-activated protein kinase 1A; OTTHUMP00000004113; p90 RSK1; p90-RSK 1; p90rsk; p90RSK1; p90S6K; pp90RSK1; Ribosomal protein S6 kinase 90kD 1; Ribosomal protein S6 kinase 90kD polypeptide 1; Ribosomal protein S6 kinase 90kDa polypeptide 1; Ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase polypeptide 1; Ribosomal S6 kinase 1; RPS6K1 alpha; rps6ka; Rps6ka1; RSK 1; RSK 1 p90; RSK; RSK-1; RSK1; S6K alpha 1; S6K-alpha-1. |
DATASHEET
Host:Rabbit
Target Protein:RPS6KA1
IR:Immunogen Range:51-150/735
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6195
Swiss Prot:Q15418
Source:KLH conjugated synthetic peptide derived from human RPS6KA1:51-150/735
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Rsk1 is a member of a family of 90kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RPS6KA1) Polyclonal Antibody, Unconjugated (bs-10365R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RPS6KA1) Polyclonal Antibody, Unconjugated (bs-10365R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RPS6KA1) Polyclonal Antibody, Unconjugated (bs-10365R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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