Anti-FUT10 antibody (bs-16197R)

Anti-FUT10 antibody

DATASHEET

Host:Rabbit

Target Protein:FUT10

IR:Immunogen Range:381-479/479

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:84750

Swiss Prot:Q6P4F1

Source:KLH conjugated synthetic peptide derived from human FUT10:381-479/479 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Glycosyltransferases that mediate the regio- and stereoselective transfer of sugars, such as the fucosyltransferases, determine cell surface-carbohydrate profiles, which is an essential interface for biological recognition processes. Fucosyltransferases catalyze the covalent association of fucose to different positional linkages in sugar acceptor molecules. Hematopoietic lineages rely on Fucosyltransferases to confer a surface carbohydrate phenotype, which mediates proper cell adhesion, molecule recruitment and cell trafficking. Localized to the Golgi apparatus as a single-pass transmembrane protein, FucT-X, also designated ?(1,3)-fucosyltransferase 10 or FUT10, is a 479 amino acid protein that is involved in protein modification and glycosylation. There are seven isoforms of FucT-X that are produced as a result of alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Sheep
Chimpanzee
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: PC-3 (human)cell Lysate at 40 ug
Primary: Anti- FUT10 (bs-16197R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-FUT10 (bs-16197R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT10) Polyclonal Antibody, Unconjugated (bs-16197R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.