Anti-GAL3ST4 antibody (bs-16215R)

Anti-GAL3ST4 antibody

DATASHEET

Host:Rabbit

Target Protein:GAL3ST4

IR:Immunogen Range:401-486/486

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:79690

Swiss Prot:Q96RP7

Source:KLH conjugated synthetic peptide derived from human GAL3ST4:401-486/486 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the galactose-3-O-sulfotransferase protein family. The product of this gene catalyzes sulfonation by transferring a sulfate to the C-3' position of galactose residues in O-linked glycoproteins. This enzyme is highly specific for core 1 structures, with asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc being good substrates. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: placenta (mouse) Lysate at 40 ug
Primary: Anti-GAL3ST4(bs-16215R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Sample: pancreas (mouse) Lysate at 40 ug
Primary: Anti-GAL3ST4(bs-16215R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Tissue/cell: Rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min;
Incubation: Anti-GAL3ST4 Polyclonal Antibody, Unconjugated(bs-16215R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining