Anti-Phospho-Insulin Receptor (Tyr999) antibody (bs-16680R)

Anti-Phospho-Insulin Receptor (Tyr999)  antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-Insulin Receptor (Tyr999)

IR:Immunogen Range:PE(p-Y)LS

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3643

Swiss Prot:P06213

Source:KLH conjugated synthesised phosphopeptide derived from human Insulin Receptor around the phosphorylation site of Tyr999:PE(p-Y)LS 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Rat
Chicken
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lovo (Human)Cell Lysate at 40 ug
HepG2 (Human) Cell Lysate at 40 ug
Primary: Anti-p-IFNGR1 (Tyr457)(bs-16680R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 70kD
Observed band size: 125kD

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Insulin Receptor (Tyr999)) Polyclonal Antibody, Unconjugated (bs-16680R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.