Anti-ODCp antibody (bs-16872R)

Anti-ODCp antibody 

DATASHEET

Host:Rabbit

Target Protein:ODCp

IR:Immunogen Range:51-150/460

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:113451

Swiss Prot:Q96A70

Source:KLH conjugated synthetic peptide derived from human ODCp:51-150/460 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ODCp is a 460 amino acid protein that is expressed in brain and testis. ODCp is a member of the Orn/Lys/Arg decarboJNClase class-II family that catalyzes the creation of CO2 and agmatine from L-arginine. Mammalian ODCp differs from the forms expressed in bacteria and plants and shares less than 50% homology with Ornithine decarboJNClase (ODC). ODCp is associated with the mitochondrial membrane where excess agmatine can be degraded by the enzyme Agmatinase or bound by the imidazoline recepto. In the brain, the highest levels of ODCp are found in the hypothalamus. Mammalian ODCp is thermally unstable and can be inhibited by Ca2+, Co2+ and polyamines.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Testis (Mouse)Lysate at 40 ug
Primary: Anti-ODCp(bs-16872R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50kD

Sample: Raji (Human)Cell Lysate at 40 ug
Primary: Anti-ODCp(bs-16872R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODCp) Polyclonal Antibody, Unconjugated (bs-16872R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.