Anti-KIAA0513 antibody (bs-16975R)

Anti-KIAA0513 antibody

DATASHEET

Host:Rabbit

Target Protein:KIAA0513

IR:Immunogen Range:21-100/411

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9764

Swiss Prot:O60268

Source:KLH conjugated synthetic peptide derived from human KIAA0513:21-100/411 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Cerebellum (Mouse)Lysate at 40 ug
Primary: Anti-KIAA0513(bs-16975R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 47kD
Observed band size: 47kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIAA0513) Polyclonal Antibody, Unconjugated (bs-16975R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIAA0513) Polyclonal Antibody, Unconjugated (bs-16975R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.