Anti-KIAA0701 antibody (bs-16980R)

Anti-KIAA0701 antibody

DATASHEET

Host:Rabbit

Target Protein:KIAA0701

IR:Immunogen Range:1-100/1464

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:23074

Swiss Prot:A0JNW5

Source:KLH conjugated synthetic peptide derived from human KIAA0701:1-100/1464 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:UHRF1BP1L (UHRF1 Binding Protein 1 Like) is a Protein Coding gene. An important paralog of this gene is UHRF1BP1.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Protein: brain(mouse) lysate at 40ug;
Primary: rabbit Anti-KIAA0701 (bs-16980R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:161 kD
Observed band size:161kD

Sample:
Stomach (Mouse) Lysate at 40 ug
Bladder (Mouse) Lysate at 40 ug
Primary: Anti- KIAA0701 (bs-16980R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 161 kD
Observed band size: 161 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KIAA0701 Polyclonal Antibody, Unconjugated(bs-16980R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining