Anti-EGFRvIII antibody (bs-10627R)

Anti-EGFRvIII antibody 

DATASHEET

Host:Rabbit

Target Protein:EGFRvIII

IR:Immunogen Range:321-520/1210

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1956

Swiss Prot:P00533

Source:KLH conjugated synthetic peptide derived from human EGFRvIII:321-520/1210 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-EGFRvIII (bs-10627R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100/130 kD
Observed band size: 120 kD

Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-EGFRvIII (bs-10627R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100/130 kD
Observed band size: 120 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti-EGFRvIII (bs-10627R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100/130 kD
Observed band size: 120 kD