Anti-phospho-JNK1 (Thr183) antibody
| 英文名称 | phospho-JNK1 (Thr183) |
| 中文名称 | 磷酸化氨基末端激酶1抗体 |
| 别 名 | JNK1 (phospho T183); p-JNK1 (phospho T183); AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK 1; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; mapk8; Mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; MK08_HUMAN; p54 gamma; Prkm8; Protein kinase JNK1; Protein kinase, mitogen-activated, 8; SAPK 1; SAPK gamma; SAPK1; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1. |
DATASHEET
Host:Rabbit
Target Protein:phospho-JNK1 (Thr183)
IR:Immunogen Range:MM(p-T)PY
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5599
Swiss Prot:P45983
Source:KLH conjugated synthesised phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183:MM(p-T)PY
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Zebrafish
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Hela-UV(Human) Cell Lysate at 30 ug
293T-UV(Human) Cell Lysate at 30 ug
Jurkat-UV(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-JNK1 (Thr183) (bs-17591R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-JNK1 (Thr183) (bs-17591R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-JNK1 (Thr183)) Polyclonal Antibody, Unconjugated (bs-17591R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
好评度