Anti-ZBTB9/ZNF919 antibody (bs-18457R)

Anti-ZBTB9/ZNF919 antibody

DATASHEET

Host:Rabbit

Target Protein:ZBTB9/ZNF919

IR:Immunogen Range:331-430/473

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:221504

Swiss Prot:Q96C00

Source:KLH conjugated synthetic peptide derived from human ZBTB9/ZNF919:331-430/473 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ZBTB9 is a 473 amino acid member of the Krüppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZBTB9 contains a BTB domain, also known as a POZ domain, which inhibits DNA binding and mediates homotypic and heterotypic dimerization. Characteristics of the BTB domain suggest that ZBTB43 functions as a transcription regulator.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Du145(Human) Cell Lysate at 40 ug
Lovo(Human) Cell Lysate at 40 ug
Primary: Anti-ZBTB9/ZNF919 (bs-18457R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZBTB9/ZNF919) Polyclonal Antibody, Unconjugated (bs-18457R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZBTB9/ZNF919) Polyclonal Antibody, Unconjugated (bs-18457R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.