Anti-TMEM175 antibody (bs-18922R)

Anti-TMEM175 antibody

DATASHEET

Host:Rabbit

Target Protein:TMEM175

IR:Immunogen Range:231-330/504

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:84286

Swiss Prot:Q9BSA9

Source:KLH conjugated synthetic peptide derived from human TM175:231-330/504 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:TMEM175 (Transmembrane Protein 175) is a Protein Coding gene.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Liver (Mouse) Lysate at 40 ug
Lane 4: Testis (Mouse) Lysate at 40 ug
Lane 5: Lung (Mouse) Lysate at 40 ug
Lane 6: Spleen (Mouse) Lysate at 40 ug
Lane 7: Cerebral cortex (Rat) Lysate at 40 ug
Lane 8: Liver (Rat) Lysate at 40 ug
Lane 9: Testis (Rat) Lysate at 40 ug
Primary: Anti-TMEM175 (bs-18922R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56/43 kD
Observed band size: 45 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM175) Polyclonal Antibody, Unconjugated (bs-18922R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM175) Polyclonal Antibody, Unconjugated (bs-18922R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.