Anti-Secretogranin V antibody
| 英文名称 | Secretogranin V |
| 中文名称 | 神经内分泌颗粒蛋白5抗体 |
| 别 名 | 7B2; 7B2 protein; APPG; Neuroendocrine protein 7B2; P7B2; Pituitary polypeptide; Pituitary polypeptide; Prohormone convertase chaperone; SCG 5; SCG5; Secretogranin 5; Secretogranin V 7B2 protein; Secretogranin5; SecretograninV; Secretory granule endocrine protein I; Secretory granule endocrine protein I; Secretory granule neuroendocrine protein 1 7B2 protein; Secretory granule neuroendocrine protein 1; Sg V; SGNE 1; SGNE1; SgV; 7B2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Secretogranin V
IR:Immunogen Range:1-100/212
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6447
Swiss Prot:P05408
Source:KLH conjugated synthetic peptide derived from human PHF21A:1-100/212
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Sg V is a 212 amino acid protein that is secreted by endocrine and neuroendocrine secretory granules and belongs to the 7B2 family. Existing as two alternatively spliced isoforms, Sg V interacts with PC2 and, via this interaction, functions as a molecular chaperone for PC2, effectively preventing its premature activation in regulated secretory pathways. More specifically, Sg V binds to PC2 and facilitates its transport from the endoplasmic reticulum to secretory compartments, thus allowing PC2 to be cleaved and activated during the correct phase of the regulated secretory pathway. Sg V is subject to post-translational sulfation on specific tyrosine residues and is underexpressed in medulloblastomas, suggesting a role in tumor suppression. The gene encoding Sg V maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Secretogranin V(bs-10762R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/21 kD
Observed band size: 21 kD
Protein: spleen(mouse) lysate at 40ug;
Primary: rabbit Anti-Secretogranin V (bs-10762R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:17/21 kD
Observed band size:21 kD
Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Secretogranin V) Polyclonal Antibody, Unconjugated (bs-10762)t 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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