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Anti-NSUN6 antibody (bs-19484R)

Anti-NSUN6 antibody (bs-19484R)

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Anti-NSUN6 antibody

英文名称NSUN6
中文名称NSUN6蛋白抗体
别    名4933414E04Rik; FLJ23743; NOL1/NOP2/Sun and PUA domain-containing protein 1; NOL1/NOP2/Sun domain family 6; NOL1/NOP2/Sun domain family member 6; NOP2; NOPD1; NSUN6; NSUN6_HUMAN; nucleolar protein (NOL1/NOP2/sun) and PUA domains 1; Putative methyltransferase NSUN6; Sun domain family, member 6.  

DATASHEET

Host:Rabbit

Target Protein:NSUN6

IR:Immunogen Range:51-150/469

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:221078

Swiss Prot:Q8TEA1

Source:KLH conjugated synthetic peptide derived from human NSUN6:51-150/469 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Hela (human)Cell Lysate at 40 ug
Primary: Anti-NSUN6(bs-19484R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD

Sample:
K562 Cell (Human) Lysate at 30 ug
Primary: Anti- NSUN6 (bs-19484R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD

Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-NSUN6 Polyclonal Antibody, Unconjugated(bs-19484R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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