Anti-phospho-Parkin (Ser65) antibody
| 英文名称 | phospho-Parkin (Ser65) |
| 中文名称 | 磷酸化帕金森病蛋白2抗体 |
| 别 名 | Parkin (phospho S65); p-Parkin (phospho S65); AR JP; E3 ubiquitin ligase; E3 ubiquitin protein ligase parkin; E3 ubiquitin-protein ligase parkin; FRA6E; LPRS 2; LPRS2; PARK 2; PARK2; Parkin 2; Parkinson disease (autosomal recessive juvenile) 2; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; Parkinson protein 2 E3 ubiquitin protein ligase; Parkinson protein 2, E3 ubiquitin protein ligase (parkin); PDJ; PRKN 2; PRKN; PRKN2; PRKN2_HUMAN; Ubiquitin E3 ligase PRKN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-Parkin (Ser65)
IR:Immunogen Range:QQ(p-S)IV
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5071
Swiss Prot:O60260
Source:KLH conjugated synthesised phosphopeptide derived from human Parkin around the phosphorylation site of Ser65:QQ(p-S)IV
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Pig
Cow
Horse
Sheep
Monkey
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Thymus (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Skeletal muscle (Mouse) Lysate at 40 ug
Primary: Anti- phospho-Parkin (Ser65) (bs-19882R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample: A673（Human）Cell Lysate at 40 ug
Primary: Anti-p-Parkin(Ser65) (bs-10196R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Parkin (Ser65)) Polyclonal Antibody, Unconjugated (bs-19882R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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