Anti-PRKRIR antibody
| 英文名称 | PRKRIR |
| 中文名称 | 蛋白激酶PKR抑制蛋白PRKRIR抗体 |
| 别 名 | 52 kDa repressor of the inhibitor of the protein kinase; 58 kDa interferon induced protein kinase interacting protein; DAP4; Death associated protein 4; Inhibitor of protein kinase PKR; Interferon inducible double stranded RNA dependent inhibitor; MGC102750; P52rIPK; P58 repressor; p58IPK interacting protein; THAP domain containing protein 0; THAP0. P52K_HUMAN |
DATASHEET
Host:Rabbit
Target Protein:PRKRIR
IR:Immunogen Range:301-400/761
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5612
Swiss Prot:O43422
Source:KLH conjugated synthetic peptide derived from human PRKRIR:301-400/761
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:PRKRIR is upstream regulator of interferon induced serine/threonine protein kinase R (PKR). It may block the PKR inhibitory function of P58IPK, resulting in the restoration of kinase activity and suppression of cell growth.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-PRKRIR (bs-19963R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRKRIR) Polyclonal Antibody, Unconjugated (bs-19963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRKRIR) Polyclonal Antibody, Unconjugated (bs-19963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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