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Anti-PPP2R2C antibody (bs-19547R)

Anti-PPP2R2C antibody (bs-19547R)

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Anti-PPP2R2C antibody 

英文名称PPP2R2C
中文名称蛋白磷酸酶2调节亚基2C抗体
别    名2ABG_HUMAN; B55 gamma; Gamma isoform of regulatory subunit B55 protein phosphatase 2; IMYPNO 1; IMYPNO; IMYPNO1; MGC33570; OTTHUMP00000115505; Phosphoprotein phosphatase 2A BR gamma regulatory chain; PP2A subunit B B gamma isoform; PP2A subunit B B55 gamma isoform; PP2A subunit B isoform B55-gamma; PP2A subunit B isoform gamma; PP2A subunit B isoform PR55-gamma; PP2A subunit B isoform R2-gamma; PP2A subunit B PR55 gamma isoform; PP2A subunit B R2 gamma isoform; PPP2R2C; PPP2R2C protein; PR 52; PR 55G; PR52; PR55G; Protein phosphatase 2 (formerly 2A) regulatory subunit B gamma isoform; Protein phosphatase 2 regulatory subunit B gamma; Protein phosphatase 2 regulatory subunit B gamma isoform; Protein phosphatase 2A1 B gamma subunit; Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B gamma isoform; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B gamma isoform.  

DATASHEET

Host:Rabbit

Target Protein:PPP2R2C

IR:Immunogen Range:1-100/447

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5522

Swiss Prot:Q9Y2T4

Source:KLH conjugated synthetic peptide derived from human PPP2R2C:1-100/447 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Rat) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-PPP2R2C (bs-19547R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R2C) Polyclonal Antibody, Unconjugated (bs-19547R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R2C) Polyclonal Antibody, Unconjugated (bs-19547R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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