Anti-PDE1A antibody
| 英文名称 | PDE1A |
| 中文名称 | 磷酸二酯酶1A抗体 |
| 别 名 | 3' 5' cyclic nucleotide phosphodiesterase; 5''-cyclic nucleotide phosphodiesterase 1A; 61 kDa Cam PDE; 61 kDa Cam-PDE; Calcium/calmodulin dependent 3' 5' cyclic nucleotide phosphodiesterase 1A; Calcium/Calmodulin stimulated cyclic nucleotide phosphodiesterase; Calcium/calmodulin-dependent 3''; Calmodulin-dependent Phosphodiesterase; Cam PDE 1A; Cam-PDE 1A; CAM-PDE-1A; HCAM 1; hCam-1; HCAM1; HSPDE1A; MGC26303; PDE 1A; PDE1A; PDE1A_HUMAN; Phosphodiesterase 1A; Phosphodiesterase 1A calmodulin dependent. |
DATASHEET
Host:Rabbit
Target Protein:PDE1A
IR:Immunogen Range:451-535/535
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5136
Swiss Prot:P54750
Source:KLH conjugated synthetic peptide derived from human PDE1A:451-535/535
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-PDE1A (bs-9967R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDE1A) Polyclonal Antibody, Unconjugated (bs-9967R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDE1A) Polyclonal Antibody, Unconjugated (bs-9967R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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