Anti-PLVAP antibody (bs-12737R)

Anti-PLVAP antibody

DATASHEET

Host:Rabbit

Target Protein:PLVAP

IR:Immunogen Range:301-400/442

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:83483

Swiss Prot:Q9BX97

Source:KLH conjugated synthetic peptide derived from human PLVAP:301-400/442 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:PLVAP (Plasmalemma Vesicle Associated Protein) is a Protein Coding gene. Diseases associated with PLVAP include Breast Benign Neoplasm and Thoracic Benign Neoplasm. GO annotations related to this gene include protein homodimerization activity.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
kidney(mouse) Lysate at 40 ug
Primary: Anti- PLVAP(bs-12737R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 55kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (bs-12737R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (bs-12737R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.