Anti-ZNF775 antibody (bs-4382R)

Anti-ZNF775 antibody

DATASHEET

Host:Rabbit

Target Protein:ZNF775

IR:Immunogen Range:61-160/537

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:285971

Swiss Prot:Q96BV0

Source:KLH conjugated synthetic peptide derived from human ZNF775:61-160/537 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ZNF775 belongs to the krueppel C2H2 type zinc finger protein family. It contains 11 C2H2 type zinc fingers. ZNF775 may be involved in transcriptional regulation.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- ZNF775 (bs-4382R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF775) Polyclonal Antibody, Unconjugated (bs-4382R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF775) Polyclonal Antibody, Unconjugated (bs-4382R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.