Anti-PTPLB antibody
| 英文名称 | PTPLB |
| 中文名称 | 蛋白酪氨酸磷酸酶PTPLB抗体 |
| 别 名 | 3 hydroJNCacyl CoA dehydratase 2; 3-hydroJNCacyl-CoA dehydratase 2; HACD2; HACD2_HUMAN; Protein tyrosine phosphatase like (proline instead of catalytic arginine) member b; Protein tyrosine phosphatase like member B; Protein-tyrosine phosphatase-like member B; PTPLB; Very-long-chain (3R)-3-hydroJNCacyl-[acyl-carrier protein] dehydratase 2. |
DATASHEET
Host:Rabbit
Target Protein:PTPLB
IR:Immunogen Range:181-254/254
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:201562
Swiss Prot:Q9D3B1
Source:KLH conjugated synthetic peptide derived from human PTPLB:181-254/254
Purification:affinity purified by Protein A
Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene can catalyze the third step (dehydration) in the conversion of long chain fatty acids to very long chain fatty acids. The encoded protein localizes to the endoplasmic reticulum membrane. [provided by RefSeq, Jul 2016]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Du145(Human) Cell Lysate at 30 ug
Primary: Anti-PTPLB (bs-4429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Sample:
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-PTPLB (bs-4429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTPLB) Polyclonal Antibody, Unconjugated (bs-4429R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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