Anti-phospho-beta Catenin (Ser37) antibody (bs-20603R)

Anti-phospho-beta Catenin (Ser37) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-beta Catenin (Ser37)

IR:Immunogen Range:IH(p-S)GA

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1499

Swiss Prot:P35222

Source:KLH conjugated synthesised phosphopeptide derived from human beta Catenin around the phosphorylation site of Ser37:IH(p-S)GA 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009].

Size:100ul

Concentration:1mg/ml

Applications:IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-beta Catenin (Ser37)) Polyclonal Antibody, Unconjugated (bs-20603R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-beta Catenin (Ser37)) Polyclonal Antibody, Unconjugated (bs-20603R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Blank control(blue):Hela (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 0.3%tritionx-100 for 5 min at room temperature).
Primary Antibody:Rabbit Anti-phospho-beta Catenin (Ser37) antibody (bs-20603R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-FITC), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.