Anti-Vimentin antibody (bs-23064R)

Anti-Vimentin antibody 

DATASHEET

Host:Rabbit

Target Protein:Vimentin

IR:Immunogen Range:151-250/466

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7431

Swiss Prot:P08670

Source:KLH conjugated synthetic peptide derived from human Vimentin:151-250/466 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Spleen (Rat) Lysate at 40 ug
Primary: Anti- Vimentin (bs-23064R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

Sample: U87MG(human) Cell Lysate at 40 ug
Primary: Anti- Vimentin (bs-23064R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

Sample:
U87MG (Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
Primary: Anti-Vimentin (bs-23064R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 53 kD