Anti-CD163 antibody (bs-23127R)

Anti-CD163 antibody

DATASHEET

Host:Rabbit

Target Protein:CD163

IR:Immunogen Range:551-650/1156

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9332

Swiss Prot:Q86VB7

Source:KLH conjugated synthetic peptide derived from human CD163:551-650/1156 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CD163 is a 130 kDa membrane glycoprotein. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CD163 (bs-23127R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 130 kD

Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: HL60 (Human) Cell Lysate at 30 ug
Primary: Anti-CD163 (bs-23127R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kD
Observed band size: 110 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-CD163 (bs-23127R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 130 kD