Anti-HRH3 antibody (bs-23318R)

Anti-HRH3 antibody

DATASHEET

Host:Rabbit

Target Protein:HRH3

IR:Immunogen Range:281-380/445

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:11255

Swiss Prot:Q9Y5N1

Source:KLH conjugated synthetic peptide derived from human HRH3:281-380/445 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Jurkat(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
Primary: Anti-HRH3 (bs-23318R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD

Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-HRH3 (bs-23318R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD

Sample:
Brain (Rat) Lysate at 40 ug
Primary: Anti-HRH3 (bs-23318R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD