Anti-DNase II antibody
| 英文名称 | DNase II |
| 中文名称 | 脱氧核糖核酸酶2抗体 |
| 别 名 | Acid DNase; DeoJNCribonuclease 2 alpha; DeoJNCribonuclease 2; DeoJNCribonuclease II alpha; DeoJNCribonuclease II; DeoJNCribonuclease II lysosomal; DeoJNCribonuclease-2-alpha; DeoJNCribonuclease2; DeoJNCribonucleaseII; DNASE 2; DNASE 2A; DNase II alpha; DNase II lysosomal; DNASE2; DNASE2A; DNaseII; DNL 2; DNL; DNL2; DNS2A_HUMAN; Lysosomal DNase II; R31240 2; R31240_2. |
DATASHEET
Host:Rabbit
Target Protein:DNase II
IR:Immunogen Range:201-300/360
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1777
Swiss Prot:O00115
Source:KLH conjugated synthetic peptide derived from human DNase II:201-300/360
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-DNase II (bs-23335R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 53 kD
Sample:
Hela Cell Lysate at 30 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-DNase II (bs-23335R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 53 kD
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Stomach (Mouse) Lysate at 40 ug
Lane 3: Liver (Mouse) Lysate at 40 ug
Lane 4: Kidney (Mouse) Lysate at 40 ug
Lane 5: Testis (Mouse) Lysate at 40 ug
Lane 6: Cerebrum (Rat) Lysate at 40 ug
Lane 7: Liver (Rat) Lysate at 40 ug
Lane 8: Kidney (Rat) Lysate at 40 ug
Lane 9: A431 (Human) Cell Lysate at 30 ug
Lane 10: Huvec (Human) Cell Lysate at 30 ug
Primary:
Anti-DNase II (bs-23335R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
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