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Anti-Peroxiredoxin-6 antibody (bs-23508R)

Anti-Peroxiredoxin-6 antibody (bs-23508R)

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Anti-Peroxiredoxin-6 antibody

英文名称Peroxiredoxin-6
中文名称过氧化物还原酶6抗体
别    名1 Cys antibody; 1 Cys peroxiredoxin; 1 Cys PRX; 1 cysPrx; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; 9430088D19Rik; AA690119; Acidic calcium independent phospholipase A2; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; AOP2; Aop2 rs3; Brp 12; Ciliary body glutathione peroxidase; CP 3; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1. ; Epididymis secretory sperm binding protein Li 128m ; GPx; HEL S 128m ; KIAA0106; Liver 2D page spot 40; Ltw4; Lvtw 4 ; MGC46173; mKIAA0106; Non selenium glutathione peroxidase; Non-selenium glutathione peroxidase; NSGPx; ORF06; OTTHUMP00000032693; p29; Peroxiredoxin6; PHGPx; Phospholipase A2 lysosomal; PLA2; PRDX 6; PRDX6; Prdx6 rs3; PRDX6_HUMAN; PRX; Red blood cells page spot 12; Thiol specific antioxidant protein;  

DATASHEET

Host:Rabbit

Target Protein:Peroxiredoxin-6

IR:Immunogen Range:151-224/224

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9588

Swiss Prot:P30041

Source:KLH conjugated synthetic peptide derived from human Peroxiredoxin-6:151-224/224 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lung (Rat) Lysate at 40 ug
Primary: Anti-Peroxiredoxin-6 (bs-23508R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD

Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peroxiredoxin-6) Polyclonal Antibody, Unconjugated (bs-23508R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peroxiredoxin-6) Polyclonal Antibody, Unconjugated (bs-23508R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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