Anti-SHISA2 antibody (bs-24152R)

Anti-SHISA2  antibody

DATASHEET

Host:Rabbit

Target Protein:SHISA2

IR:Immunogen Range:1-100/235

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:66940

Swiss Prot:Q9D7I0

Source:KLH conjugated synthetic peptide derived from mouse SHISA2 :1-100/235 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the shisa family. The encoded protein is localized to the endoplasmic reticulum, and together with p53 induces apoptosis in a caspase-dependent manner. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Spleen (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-SHISA2 (bs-24152R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD

Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-SHISA2 (bs-24152R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SHISA2) Polyclonal Antibody, Unconjugated (bs-24152R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.