Anti-ZNF423 antibody (bs-22985R)

Anti-ZNF423 antibody

DATASHEET

Host:Rabbit

Target Protein:ZNF423

IR:Immunogen Range:191-290/1284

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:23090

Swiss Prot:Q2M1K9

Source:KLH conjugated synthetic peptide derived from human ZNF423 :191-290/1284 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:OAZ is a 30-zinc finger, DNA-binding factor that associates with members of the Smad family of transcription factors in response to BMP2 activation. Bone morphogenic proteins (BMPs), are the largest group within the TGF∫ growth factors superfamily and are involved in embryonic development, specifically the formation of left-right asymmetry, neurogenesis, organogenesis and skeletal development. BMPs bind to surface receptors, which then phosphorylate serine residues of specific Smad proteins to induce Smad translocation to the nucleus and transcriptional activation of BMP targeted genes. OAZ specifically cooperates with the BMP-activated Smads, namely Smad1, 5 and 8, in binding to the CAGAC and TGGAGC boxes within the BRE, or BMP response element, and activating transcription. OAZ contains a BMP signaling module formed by two clusters of fingers that individually associate with either the Smads or the BMP response element. Distinct regions of OAZ, separate from the modules involved in BMP regulation, also enable OAZ to function as a transcriptional partner of Olf-1/EBF in olfactory epithelium and lymphocyte development, indicating that, as a multi–zinc finger protein, OAZ may have dual roles in signal transduction during development.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:400-800)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Cerebellum (Rat) Lysate at 40 ug
Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-ZNF423 (bs-22985R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 145/138 kD
Observed band size: 145 kD

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF423) Polyclonal Antibody, Unconjugated (bs-22985R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF423) Polyclonal Antibody, Unconjugated (bs-22985R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.