Anti-TAGLN2 antibody (bs-55211R)

Anti-TAGLN2 antibody

DATASHEET

Host:Rabbit

Target Protein:TAGLN2

IR:Immunogen Range:1-199/199

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:8407

Swiss Prot:P37802

Source:Recombinant human TAGLN2:1-199/199 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Background:The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ICC(1:100)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: 293T (Human) Cell Lysate at 25 ug
Lane 2: TAGLN2 knockout (KO) 293T (Human) Cell Lysate at 25 ug
Primary: Anti-TAGLN2 (bs-55211R) at 1/1000 dilution
Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution
Predicted band size: 18 kD
Observed band size: 20 kD

Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Placenta (Mouse) Lysate at 40 ug
Lane 3: Uterus (Mouse) Lysate at 40 ug
Lane 4: U251 (Human) Cell Lysate at 30 ug
Lane 5: A431 (Human) Cell Lysate at 30 ug
Lane 6: U87MG (Human) Cell Lysate at 30 ug
Lane 7: U2os (Human) Cell Lysate at 30 ug
Lane 8: Huvec (Human) Cell Lysate at 30 ug
Lane 9: A673 (Human) Cell Lysate at 30 ug
Primary:
Anti-TAGLN2 (bs-55211R) at 1/1000 dilution
Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 22 kD

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)TAGLN2 polyclonal Antibody, Unconjugated (bs-55211R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.