Anti-HIF Prolyl HydroJNClases antibody
| 英文名称 | HIF Prolyl HydroJNClases |
| 中文名称 | 缺氧诱导因子脯氨酰4羟化酶抗体 |
| 别 名 | PHD4/prolyl hydroJNClase; EGLN4; FLJ20262; HIF prolyl hydroJNClase PH4; HIF-PH4; HIF-prolyl hydroJNClase 4; HIFPH4; HPH-4; Hypoxia inducible factor prolyl 4 hydroJNClase; Hypoxia inducible factor prolyl hydroJNClase; Hypoxia-inducible factor prolyl hydroJNClase 4; P4H TM; P4H with transmembrane domain; P4H-TM; P4htm; P4HTM_HUMAN; PH 4; PH4; PHD4; Proline 4 hydroJNClase; Prolyl 4 hydroJNClase transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain containing 4; Transmembrane prolyl 4-hydroJNClase. |
DATASHEET
Host:Rabbit
Target Protein:HIF Prolyl HydroJNClases
IR:Immunogen Range:201-300/502
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:54681
Swiss Prot:Q9NXG6
Source:KLH conjugated synthetic peptide derived from human PH-4:201-300/502
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The product of this gene belongs to the family of prolyl 4-hydroJNClases. This protein is a prolyl hydroJNClase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oJNCgen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Muscle (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- HIF Prolyl HydroJNClases (bs-1871R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF Prolyl HydroJNClases) Polyclonal Antibody, Unconjugated (bs-1871R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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