Anti-CyclooJNCgenase 1 antibody
| 英文名称 | CyclooJNCgenase 1 |
| 中文名称 | 前列腺素氧化环化酶1抗体 |
| 别 名 | CyclooJNCgenase-1; COX-1; COX 1; COX1; CyclooJNCgenase1; Prostaglandin-endoperoxide synthase-1; prostaglandin-endoperoxide synthase 1; PTGS-1; Prostaglandin G/H synthase 1 precursor; prostaglandin G/H synthase 1 isoform 1 precursor; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; PGH1_HUMAN; PTGS1; Prostaglandin G/H synthase 1 precursor. |
DATASHEET
Host:Rabbit
Target Protein:CyclooJNCgenase 1
IR:Immunogen Range:151-250/599
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5742
Swiss Prot:Q63921
Source:KLH conjugated synthetic peptide derived from human CyclooJNCgenase 1:151-250/599
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Huvec(Human) Cell Lysate at 30 ug
Primary: Anti-CyclooJNCgenase 1 (bs-0582R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COX-1 Polyclonal Antibody, Unconjugated(bs-0582R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
好评度