Anti-Dopamine beta HydroJNClase antibody (bs-0596R)

Anti-Dopamine beta HydroJNClase antibody

DATASHEET

Host:Rabbit

Target Protein:Dopamine beta HydroJNClase

IR:Immunogen Range:471-550/621

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1621

Swiss Prot:P09172

Source:KLH conjugated synthetic peptide derived from human DBH:471-550/621 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Dopamine-beta-HydroJNClase (DBH), Dopamine can be transformed into Noradrenalin. Conversion of dopamine to noradrenaline. [SUBUNIT] Homotetramer composed of two non-covalently bound disulfide-linked dimers. [SUBCELLULAR LOCATION] Soluble dopamine beta-hydroJNClase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (Potential). Belongs to the copper type II ascorbate-dependent monooJNCgenase family.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Jurkat (Human) Cell Lysate at 40 ug
Primary: Anti-Dopamine beta HydroJNClase (bs-0596R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOPO) Polyclonal Antibody, Unconjugated (bs-0596R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.