Anti-SCARB1/Scavenger Receptor BI antibody (bs-1186R)

Anti-SCARB1/Scavenger Receptor BI antibody

DATASHEET

Host:Rabbit

Target Protein:SCARB1/Scavenger Receptor BI

IR:Immunogen Range:21-100/552

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:949

Swiss Prot:Q8WTV0

Source:KLH conjugated synthetic peptide derived from human CD36L1:21-100/552 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BI (Scavenger Receptor BI) protein binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism. In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland. Scavenging Receptor SR-BI plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Ovary (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti-SCARB1/Scavenger Receptor BI (bs-1186R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 76 kD

Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-SCARB1/Scavenger Receptor BI (bs-1186R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 76 kD

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-SCARB1 antibody (bs-1186R-FITC) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.