Anti-His tag antibody (bs-0287R)

Anti-His tag antibody 

DATASHEET

Host:Rabbit

Target Protein:His tag

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:N/A

Swiss Prot:N/A

Source:Synthetic peptide: HHHHHH conjugated to KLH: 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboJNC- termini of targeted proteins in transfected or transformed cells.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:1000-5000)

Cross Reactive Species:Recombinant protein.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-His tag (bs-0287R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

Sample:
Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Primary: Anti-His tag (bs-0287R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD