Anti-Neurotrophin 4 antibody
| 英文名称 | Neurotrophin 4 |
| 中文名称 | 神经生长因子4/5抗体 |
| 别 名 | GLC1O; Neurotrophic factor 4; Neurotrophic factor 5; Neurotrophin 4/5; Neurotrophin 5 (neurotrophin 4/5); Neurotrophin 5; Neurotrophin-4; Neurotrophin-5; Neurotrophin4; Neurotrophin4/5; Neurotrophin5; Neutrophic factor 4; Neutrophic factor 5; NT 4; NT-4; NT 4/5; NT-4/5; NT 5; NT-5; NT4; NT4/5; NT4P; NT5; NTF 4; NTF 5; NTF4; NTF4_HUMAN; NTF5 |
DATASHEET
Host:Rabbit
Target Protein:Neurotrophin 4
IR:Immunogen Range:121-210/210
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4909
Swiss Prot:P34130
Source:KLH conjugated synthetic peptide derived from human NT-4:121-210/210
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Neurotrophin 4 (NT4; synonymous with NT5) belongs to the NGF beta family and is a survival factor for peripheral sensory sympathetic neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. NT4 levels are highest in the prostate, with lower levels in thymus, placenta, and skeletal muscle. NT4 is also expressed in embryonic and adult tissues.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: DU145 Cell (Human) Lysate at 40 ug
Primary: Anti-Neurotrophin 4 (bs-0158R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14/27 kD
Observed band size: 26 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Neurotrophin 4/NT-4/NT-5 Polyclonal Antibody, Unconjugated(bs-0158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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