Anti-Neurturin antibody (bs-0073R)

Anti-Neurturin antibody

DATASHEET

Host:Rabbit

Target Protein:Neurturin

IR:Immunogen Range:151-197/197

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4902

Swiss Prot:Q99748

Source:KLH conjugated synthetic peptide derived from human Neurturin:151-197/197 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Human Neurturin (NTN) and Human GDNF comprise a family of TGF-beta related neurotrophic factor which have trophic influences on a variety of neuronal populations. Neurturin promotes the survival of certain sympathetic and sensory neurins through interaction with distinct set of GDNF-like receptors

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Placenta(Mouse)lysate at 30 ug;
Brain(Mouse) lysate at 30 ug;
Primary: Anti- Neurturin (bs-0073R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 21 kD
Observed band size: 21 kD

Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Neurturin Polyclonal Antibody, Unconjugated(bs-0073R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining