Anti-PEPT1 antibody (bs-0689R)

Anti-PEPT1 antibody

DATASHEET

Host:Rabbit

Target Protein:PEPT1

IR:Immunogen Range:101-200/714(chk)

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6564

Swiss Prot:Q90WH8

Source:KLH conjugated synthetic peptide derived from human PEPT1:101-200/714(chk) 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:PEPT1(Oligopeptide transporter, small intestine isoform (Peptide transporter 1) (Intestinal H(+)/peptide cotransporter) (Solute carrier family 15 member 1) Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides. May constitute a major route for the absorption of protein digestion end-products. Subcellular Location：membrane; Multi-pass membrane protein. Tissue Specificity Intestine, kidney, liver and low in brain. Belongs to the PTR2/POT transporter (TC 2.A.17) family.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(2μg/Test)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Black line : Positive blank control SH-Sy5Y); Negative blank control (A431）
Green line : Primary Antibody (Rabbit Anti-PEPT1 antibody (bs-0689R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)
SH-Sy5Y（Positive）and A431（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PEPT1 Antibody(bs-0689R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Blank control: HCCLM3(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μl in 100 μL1X PBS containing 0.5% BSA(green).