Anti-PHD3 antibody (bs-0532R)

Anti-PHD3 antibody

DATASHEET

Host:Rabbit

Target Protein:PHD3

IR:Immunogen Range:151-239/239

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:112399

Swiss Prot:Q9H6Z9

Source:KLH conjugated synthetic peptide derived from human PHD3:151-239/239 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:PHD3 (Egl nine homolog 3; Hypoxia-inducible factor prolyl hydroJNClase 3; HIF-prolyl hydroJNClase 3; HIF-PH3; HPH-1; Egln3; Prolyl hydroJNClase domain-containing protein 3;)Catalyzes the post-translational formation of 4-hydroJNCproline in hypoxia-inducible factor (HIF) alpha proteins. HydroJNClates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oJNCgen sensor and, under normoxic conditions, targets HIF through the hydroJNClation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism. [Catalytic Activity] An HIF alpha chain L-proline + 2-oxoglutarate+ O(2) = An HIF alpha chain trans-4-hydroJNC-L-proline + succinate + CO(2). [Subcellular Location] Cytoplasm. Nucleus. Widely expressed at low levels. Expressed athigher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PHD3) Polyclonal Antibody, Unconjugated (bs-0532R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PHD3) Polyclonal Antibody, Unconjugated (bs-0532R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.