Anti-MAP126 antibody (bs-0717R)

Anti-MAP126 antibody

DATASHEET

Host:Rabbit

Target Protein:MAP126

IR:Immunogen Range:1101-1193/1193

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10615

Swiss Prot:Q96R06

Source:KLH conjugated synthetic peptide derived from human SPAG5:1101-1193/1193 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:A novel gene product, hMAP126, interacts with p29 in the yeast two-hybrid assay. The subcellular distribution of hMAP126 was localized to the mitotic spindle and is phosphorylated by p34(cdc2) kinase. Human MAP126 is likely involved in the functional and dynamic regulation of mitotic spindles.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SPAG5/MAP126/Astrin Polyclonal Antibody, Unconjugated(bs-0717R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SPAG5/MAP126/Astrin Polyclonal Antibody, Unconjugated(bs-0717R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining