Anti-ZNF300 antibody (bs-0628R)

Anti-ZNF300 antibody

DATASHEET

Host:Rabbit

Target Protein:ZNF300

IR:Immunogen Range:165-250/604

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:91975

Swiss Prot:Q96RE9

Source:KLH conjugated synthetic peptide derived from human ZNF300:165-250/604 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ZNF300 contains C2H2 (or Kruppel)-type zinc finger motifs that tetrahedrally coordinate zinc ions and form a protein structure that can bind DNA and regulate transcription (Gou et al.,2004 [PubMed 14746915]). ZNF300 belongs to the krueppel C2H2 type zinc finger protein family. It contains twelve C2H2 type zinc fingers and one KRAB domain. ZNF300 has a transcriptional repressor activity. Expressed mostly in heart, skeletal muscle and brain. Isoforms 1 and 2 are highly expressed in testis. There are three named isoforms.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-ZNF300 (bs-0628R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD

Sample:
Lane1: Testis (Mouse) Lysate at 30 ug
Lane2: Small intestine (Mouse) Lysate at 30 ug
Primary: Anti-ZNF300(bs-0628R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution;
Predicted band size : 66kD
Observed band size : 70,66kD