Anti-Synaptotagmin-4 antibody (bs-1897R)

Anti-Synaptotagmin-4 antibody

DATASHEET

Host:Rabbit

Target Protein:Synaptotagmin-4

IR:Immunogen Range:301-400/425

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6860

Swiss Prot:Q9H2B2

Source:KLH conjugated synthetic peptide derived from human Synaptotagmin-4:301-400/425 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene belongs to the synaptotagmin family. Members of this family are multi-domained, integral membrane proteins of synaptic vesicles, and are thought to serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. This gene is primarily expressed in the nervous tissues. [provided by RefSeq].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane1:Brain(Mouse) Lysate at 30 ug
Lane2:Heart(Mouse) Lysate at 30 ug
Primary: Anti-SYNAPTOTAGMIN-4(bs-1897R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution;
Predicted band size : 48kD
Observed band size : 47kD

Sample:
Cerebellum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- Synaptotagmin-4 (bs-1897R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD