Anti-S100A1 antibody
| 英文名称 | S100A1 |
| 中文名称 | S100A1抗体 |
| 别 名 | S100; S-100 protein alpha subunit; S-100 protein alpha chain; Protein S100-A1; S100 calcium-binding protein A1; S100 alpha; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 Calcium Binding Protein A1; S100 calcium-binding protein A1; S100 protein alpha polypeptide; S100A; S10A1_HUMAN; S-100 α; S-100-α; S-100α. |
DATASHEET
Host:Rabbit
Target Protein:S100A1
IR:Immunogen Range:11-94/94
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6271
Swiss Prot:P23297
Source:KLH conjugated synthetic peptide derived from human S100A1:11-94/94
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-S-100/S100A1 Polyclonal Antibody, Unconjugated(bs-0850R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-S-100/S100A1 Polyclonal Antibody, Unconjugated(bs-0850R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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