Anti-STAT1 antibody
| 英文名称 | STAT1 |
| 中文名称 | 信号转导与转录激活因子1抗体 |
| 别 名 | signal transducers and activators of transcription 1; DKFZp686B04100; ISGF 3; ISGF-3; Signal transducer and activator of transcription 1 91kDa; Signal transducer and activator of transcription 1 alpha/beta; STAT 1; STAT-1; STAT 91; STAT91; Transcription factor ISGF 3 components p91 p84; ; Transcription factor ISGF-3 components p91/p84; OTTHUMP00000163552; OTTHUMP00000165046; OTTHUMP00000165047; OTTHUMP00000205845; Signal transducer and activator of transcription 1, 91kD; Signal transducer and activator of transcription 1-alpha/beta; Signal Transductor and Activator of Transcription 1; STAT1_HUMAN; CANDF7; IMD31A; IMD31B; IMD31C. |
DATASHEET
Host:Rabbit
Target Protein:STAT1
IR:Immunogen Range:251-380/750
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6772
Swiss Prot:P42224
Source:KLH conjugated synthetic peptide derived from human STAT1:251-380/750
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STAT1) Polyclonal Antibody, Unconjugated (bs-1317R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-STAT1 Polyclonal Antibody, Unconjugated(bs-1317R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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