Anti-MAPK4 antibody (bs-1319R)

Anti-MAPK4 antibody

DATASHEET

Host:Rabbit

Target Protein:MAPK4

IR:Immunogen Range:21-130/587

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5596

Swiss Prot:P31152

Source:KLH conjugated synthetic peptide derived from human MAPK4:21-130/587 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus where it phosphorylates nuclear targets. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Independently Validated Antibody, image provided by Science Direct, badge number 029727:Formalin-fixed and paraffin embedded human heart labeled with Rabbit Anti-ERK4 Polyclonal Antibody, Unconjugated (bs-1319R) at 1:250 overnight at 4C followed by conjugation to the secondary antibody and DAB staining

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAPK4 Polyclonal Antibody, Unconjugated(bs-1319R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining