Anti-Granzyme B antibody
| 英文名称 | Granzyme B |
| 中文名称 | 颗粒酶B抗体 |
| 别 名 | GranzymeB; Granzyme-B; C11; Cathepsin G-like 1; CTLA-1; Cathespin G Like 1; CCPI; CCP-1; CGL 1; CGL1; CSP B; CSPB; CTLA 1;CTLA1; CTSGL 1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T Lymphocyte Associated Serine Esterase 1; Cytotoxic T lymphocyte proteinase 2; Fragmentin 2; Fragmentin2; Granzyme 2; Granzyme2; GRB; GZMB; Cytotoxic T-lymphocyte proteinase 2; Fragmentin-2; GRAB_HUMAN; Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1); Granzyme B; Granzyme-2; GranzymeB; HLP; T cell serine protease 1 3E; T cell serine protease 1-3E; T-cell serine protease 1-3E; HLP; Human lymphocyte protein; Lymphocyte protease; Protease Serine B; SECT; AA 18-29; T cell serine protease 1. |
DATASHEET
Host:Rabbit
Target Protein:Granzyme B
IR:Immunogen Range:21-130/247
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3002
Swiss Prot:P10144
Source:KLH conjugated synthetic peptide derived from human GZMB:21-130/247
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Granzyme B Polyclonal Antibody, Unconjugated(bs-1351R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Granzyme B antibody (bs-1351R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
好评度